ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (10): 2089-2097.doi: 10.11843/j.issn.0366-6964.2016.10.018

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The Analysis of Immune Enhancement Effect of Xinjiang Wild Artemisia rupestris L.Crude Polysaccharides on Influenza Viruses Vaccines in Mice

ZHANG Ai-lian*,WANG Dan-yang,ZHAO Shu-shu,ZHAO Bing,ZHANG Fu-chun   

  1. (Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,China)
  • Received:2016-05-16 Online:2016-10-23 Published:2016-10-23

Abstract:

The immune effects of Xinjiang Wild Artemisia rupestris L.crude polysaccharides (WARCP) as an adjuvant and sparing antigen on different doses of influenza virus vaccine (IVV) were explored.ICR mice were intramuscularly immunized respectively with different doses (0.05,0.1,0.5 μg) of the IVV alone or co-administered with 300 μg WARCP at 0 d and 14 d.The growth state of mice were observed,antibody level of IgG and IgG1,IgG2a in serum were tested by ELISA;splenocyte proliferations were tested by MTT;The expression levels of CD4+IL-4,CD8+IFN-γ and CD4+CD25+Foxp3+Treg cells were tested by flow cytometry.The results showed that no significant differences in the body weight were observed between mice from different groups (P>0.05);WARCP can significantly improve the antibody level of influenza-specific IgG and IgG1,IgG2a (P<0.05);WARCP can significantly promote lymphocyte proliferation,the secretion level of IL-4 and IFN-γ (P<0.05),and significantly reduce the level of expression of Treg cells (P<0.05);In particularly,it can enhance the humoral and cellular immunity responses of low-dose IVV,indicating an at least 91% reduction in vaccine dosage by adding WARCP as adjuvant.These results indicated that adding WARCP to IVV enhanced the immune efficacy of IVV by reducing the level of Treg cells,significantly promote Th1-type immune response particularly,it can be an adjuvant with the advantages of high safety and dose-sparing.

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